KC248412
Genome And Source
Length
N/A
Type
dsDNA
GC%
N/A
Completeness
metadata_only
Country
N/A
Year
N/A
📋 Data Provenance
Genome Browser
No ORF position data available for this genome.
Curation Status
1
Isolates
1
Hosts
1
Proteins
—
Sequence
Related Viruses
No related viruses found.
Reference
Metagenomic identification, seasonal dynamics and potential transmission mechanisms of a Daphnia- associated putative RNA-DNA virus hybrid in two temperate lakes
Hewson,I. and Hairston,N.G. Jr.
Unpublished unknown
ICTV Mapping
No ICTV mapping is available or reviewed for this record.
Reviewed Evidence
Only manual-checked evidence records are displayed. Inferred records (without direct isolate linkage) are shown with a yellow badge.
Virulence / pathogenicity
Molluscan cells in culture: Primary cell cultures and cell lines Detection and Description of a Particular Ostreid Herpesvirus 1 Genotype Associated with Massive Mortality Outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008 Identificat
pathogenicity · medium
penaeicida by in situ hybridization in shrimp tissues infected by immersion challenge.
pathogenicity · medium
By screening yeast two-hybrid cDNA libraries from intestine, stomach, and
hepatopancreas of Litopenaeus vannamei, the protein with the largest increase in gene
expression in shrimp hepatopancreas in response to VPAHPND challenge was identified
and des
pathogenicity · medium
The evolution of adaptive immune systems Nucleic acid-induced antiviral immunity in invertebrates: An evolutionary perspective Pathogen recognition and innate immunity Genetic analysis of resistance to viral infection Jak-STAT pathways and transcript
pathogenicity · medium
Proteomic analysis of muscle between hybrid abalone andparental lines Haliotis gigantea Reeve and Haliotis discus hannai Ino A proteomic study on postdiapaused embryonic development of brine shrimp (Artemia franciscana) Proteomic Comparison between T
pathogenicity · medium
Pathogenicity of a baculovirus infection causing white spot syndrome in cultured penaeid shrimps in Taiwan A nonoccluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn
pathogenicity · medium
Measures for control of “bottom death” of Pacific white shrimp A new nodavirus is associated with covert mortality disease of shrimp Prevalence and distribution of covert mortality nodavirus (CMNV) in cultured crustacean Review of current disease thr
pathogenicity · medium
nov., the causal agent of nocardiosis in Pacific oysters Investigation of the relationship between the presence of a Gram-positive bacterial infection and summer mortality of the Pacific oyster, Crassostrea gigas Thunberg Bacteriological Studies on t
pathogenicity · medium
Detection of pathogens in foods: the current state-of-the-art and future directions Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases Diagnostic system for rapid and sensitive differential detection
pathogenicity · medium
conicopora) are susceptible to infection by multiple abalone herpesvirus genotypes Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene Exploring the chronic mortality affectin
pathogenicity · medium
Temperature
In
comparison
with
RT-PCR,
dot-blot
hybridization method is cumbersome and requires a high
degree of technical skill.
temperature · medium
A hybrid descriptor based QSPR model to
predict the thermal decomposition temperature of imidazolium ionic
liquids using Monte Carlo approach.
temperature · medium
The mem-
branes were prehybridized for 2 to 4 h at 52°C for enterovirus and at 58°C for
NLV and were then hybridized with digoxigenin-labelled probes at the same
temperature.
Direct isolation and identification of pathogenic viruses from oysters implicated in gastroenteritis outbreaks are hampered by inefficient methods for recovering viruses, naturally occurring PCR inhibitors, and low levels of virus contamination.
temperature · high
One half was fixed in
Davidson’s fixative for in situ hybridization and immunochemistry analysis, another
half was frozen at –20°C for PCR analysis.
Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome.
temperature · high
PCR
amplification products were confirmed by Southern blot hybridization with an
internal digoxigenin-labeled probe (5-TTAATTCCTGCAGGTTCAGG-3) at
40°C for 2 h in 5 SSC (1 SSC is 0.15 M NaCl plus 0.015 M sodium citrate) and
sequential washes down to
temperature · high
5
6
Hybridization
7
For hybridization, the PCR product was denaturated and blotted onto a positively charged
8
nylon membrane, hybridized for 2 h at 50°C.
temperature · high
For study of ALF
expression, the cells were fixed with 95% ethanol for 5 min at room temperature
and stored in 70% ethanol at 20°C until used in hybridization experiments
according to the method described by So¨derha¨ll et al.
temperature · high
The membrane was placed in
a sealed plastic bag containing pre-warmed (42°C)
186
Li et al.: Detection of PaV1 in spiny lobsters
pre-hybridization solution (Sigma-Aldrich) and incu-
bated for 30 min with gentle agitation at room temper-
ature (RT, 25
temperature · high
PCRs were performed using an initial denaturation (95°C for 10 min)
followed by 40 cycles of denaturation (95°C for 10 s), hybridization (60°C for 20 s), and elongation (72°C
for 25 s).
temperature · high
in oysters (Tiostrea chilensis), with a consideration of organelle function Replication of a herpes-like virus in larvae of the flat oyster Tiostrea chilensis at ambient temperatures Herpes-like virus infecting Pacific-oyster larvae, Creassostrea gig
In 2008 and 2009, acute mortalities occurred in France among Pacific cupped oyster, Crassostrea gigas, spat.
temperature · high
Hosts
Diagnostic And Control Records
No curated diagnostic or control records are available for this virus.
Proteins
🧬
No protein annotations available
Loading additional protein data...
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Geographic Distribution
No geographic distribution data available
Taxonomy Information
Family
Nucleocytoviricota
Host Phylum
Arthropoda
Genome Type
dsDNA
Discovery Method
metagenomic_survey
Related Isolates
| Accession | Length | GC | Completeness | Country | Year |
|---|---|---|---|---|---|
| KC248412 | N/A | N/A | metadata_only | N/A | N/A |